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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Pigments and Anti-Cholesterol Agent Production by Monascus kaoliang KB9 and Its Color Mutants in Rice Solid Cultures) ผู้เขียน: จิราวรรณ ฉายาวัฒน์,  ดร.เสรี เจริญกิจมงคล, อาจารย์ , ดร.อภิสิฏฐ์ ศงสะเสน, รองศาสตราจารย์ , ดร.บุษบา ยงสมิทธ์, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractA comparative study of four Monascus strains, (a red-pigment-producing Monascus kaoliang KB9 strain and its three color mutants of red, yellow and white) on various agar media revealed different colony and pigment characteristics. Three agar media: MYS, GYP and PDA gave clear cultural characteristics. Upon varying medium formulas, the wild type and the red mutant showed red pigmentation, while the yellow and white mutants showed deep orange and white pigmentation, respectively. All strains were grown on rice solid culture and examined for the production of pigment and anti-cholesterol agent using a spectrophotometer and a modified HPLC method, respectively. The results showed that the wild-type strain (KB9) and its red mutant (KB10M16) could produce substantial amounts of anti-cholesterol agent compared with the yellow and white mutants (KB20M10.2 and KB20M1). Due to its good growth and production of the greatest amount of anti-cholesterol agent, the wild-type KB9 strain was selected for a study on the optimization of anti-cholesterol agent fermentation. Under favorable conditions of 64% relative humidity at room temperature (28-30?C) for 5 weeks incubation, production of the anti-cholesterol agent and red pigment was high, with 17,892 mg/kg and 4,640 Unit (OD500) and 4,834 Unit (OD400) per gram dried weight, respectively. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Intraspecific Hybridization Between Color Mutants of Trichoderma harzianum by Protoplast Fusion ) ผู้เขียน:Chiradej Chamswarngt, Kihashiro Ogawa, Wanwilai Gesnarat, Nuanwan Faroongsang สื่อสิ่งพิมพ์:pdf AbstractProtoplasts of two color mutants of Trichoderma harzianwn derived from ultraviolet treatment were prepared by using lytic enzyme system (10 mg Zymolvase. 150 mg Uzukizyme, 10 mg Chitinase and 150 mg Funcelase in 3 ml water, pH 5.0) containing 0.6 M NaC 1 as an osmotic stabilizer. Fusion between protoplasts nt two color mutants was observed in the solution containing 0.05 M glycine-NaOH buffer, pH 7.5, 0.05 M CaCI . 2H-,0 and 35% polyethylene glycol ( MW. 6,000). The fused protoplasts were successfully detected at the rate of '2 % as green colonies developed on a hypersonic solid culture medium (complete medium) overlaid with hypertonic soft medium (MM with 0.5% agar). Production of well defined white and yellow sectors on tear surface of minimal medium supplemented with 0.1U (W/V) d-camphor represented the heterokaryotic usants. Green conidia were constantly obtained after transferring the green part from green-sectored colonies :.into MM agar. This indicated that protoplast fusants were heterozygous diploid. |
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